Biomarker Analysis

Results from the analysis of ADNI samples are summarized below. Anyone with an ADNI Data Archive account may view and download the analysis methods and the analyzed data. After you login, click “Download” and “Study Data” to see all relevant ADNI documents available for download.

ADNI GO and ADNI 2: Analyses of CSF Biomarkers
Leslie M Shaw and John Q Trojanowski
Aliquots of all ADNI GO plus ADNI 2 CSF samples were analyzed using the xMAP Luminex platform and Innogenetics/Fujirebio AlzBio3 immunoassay kits. In addition 28 newly thawed randomly selected replicate aliquots were tested. The ADNI GO and ADNI 2 CSF Aβ1-42, t-tau and p-tau181 dataset in .csv file format found in the ADNI data archive provide details of the analyses and the final set of results. The full ADNI GO and ADNI 2 CSF report can be downloaded from the data archive.

Redox-reactive Antiphospholipid Autoantibodies: Early Stage Alzheimer’s Disease Blood Biomarkers 
John A. McIntyre, Dawn R. Wagenknecht, Curtis J Ramsey, Paul A. Hyslop
The HLA-Vascular Biology Laboratory report their findings measuring Redox reactive autoantibodies (R-RAA) aPL in 3 cohorts of individuals: 30 patients with mild cognitive impairment, 30 patients with Alzheimer’s dementia and 30 agematched volunteer controls, all obtained from the ADNI group. The sera from the MCI population contained significantly elevated R-RAA activity in contrast to AD patient and/or control sera.

Autoantibody biomarkers for detection and diagnosis of Alzheimer’s disease
Robert G. Nagele
We employ our newly developed biomarker discovery strategy and human protein microarrays in an effort to identify autoantibody biomarkers in human serum that can accurately diagnose cases of MCI due to early stages of AD pathology. Microarrays containing 9,486 proteins were probed with individual ADNI serum samples obtained from patients with MCI that later transitioned to full-blown AD as well as corresponding controls. Arrays were scanned with a GenePix 4000B Fluorescence Scanner. Data was acquired using GenePix Pro 7, saved as Genepix Results (GPR) files and can readily be analyzed using ProtoArray® Prospector v5.2 software, a freely available data analysis tool provided Invitrogen.